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What is FASTA and how is it used?

FASTA is among the most popular formats for storing biological sequence data. Here is what it is and how it is used.

What is FASTA?

The FASTA format is a text-based file format for storing protein and nucleic acid sequences. FASTA files commonly have the .fasta or .fa file extensions.

A FASTA file can contain one or more sequences. A sequence "block" in FASTA starts with a single-line description. The description line always begins with a greater-than character (>).

The sequence description line is immediately followed by the lines of sequence data. The residues in the sequence are represented using single-letter codes.

Here is an example of one protein sequence in the FASTA format:

>FER1_ARATH
MASTALSSAIVSTSFLRRQQTPISLRSLPFANTQSLFGLKSSTARGGRVTAMATYKVKFI
TPEGEQEVECEEDVYVLDAAEEAGLDLPYSCRAGSCSSCAGKVVSGSIDQSDQSFLDDEQ
MSEGYVLTCVAYPTSDVVIETHKEEAIM

Bottom line

Whether you perform sequence analysis on a daily basis or only occasionally run BLAST, it is important that you are familiar with the FASTA format and can easily open FASTA file and navigate their contents.

See also

Big Sequence Logos
$29.99

A collection of large-format sequence logos.

language IPA Transcription Poster
$14.99

A poster featuring the phonetic transcription of the word language in the International Phonetic Alphabet (IPA).

языкознание Morphemic Analysis Poster
$14.99

A poster featuring the morphemic analysis of the Russian word языкознание.

Sulfur Hexafluoride Molecule Poster, 2D Structure, English-Labeled
$19.99

A poster featuring the 2D structure of the sulfur hexafluoride molecule.

World Map Poster, English-Labeled
$19.99

A map of the world (countries and regions).

FASTA Parse Tree Viewer

Visualize the parse tree of FASTA files.

Sequence Alignment Viewer

Open FASTA files in the browser.

GenBank to FASTA

Convert GenBank flat files to FASTA format.

FASTA Editor

A simple online editor for biological sequences in the FASTA format.

ABI to FASTA

Convert AB1 files (Applied Biosystems/ABI trace files) to FASTA format.

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