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What is FASTA and how is it used?

FASTA is among the most popular formats for storing biological sequence data. Here is what it is and how it is used.

What is FASTA?

The FASTA format is a text-based file format for storing protein and nucleic acid sequences. FASTA files commonly have the .fasta or .fa file extensions.

A FASTA file can contain one or more sequences. A sequence "block" in FASTA starts with a single-line description. The description line always begins with a greater-than character (>).

The sequence description line is immediately followed by the lines of sequence data. The residues in the sequence are represented using single-letter codes.

Here is an example of one protein sequence in the FASTA format:

>FER1_ARATH
MASTALSSAIVSTSFLRRQQTPISLRSLPFANTQSLFGLKSSTARGGRVTAMATYKVKFI
TPEGEQEVECEEDVYVLDAAEEAGLDLPYSCRAGSCSSCAGKVVSGSIDQSDQSFLDDEQ
MSEGYVLTCVAYPTSDVVIETHKEEAIM

Bottom line

Whether you perform sequence analysis on a daily basis or only occasionally run BLAST, it is important that you are familiar with the FASTA format and can easily open FASTA file and navigate their contents.

See also

Big Sequence Logos
$29.99

A collection of large-format sequence logos.

Green Fluorescent Protein Poster, Technical Illustration, English-Labeled
$19.99

A poster featuring the green fluorescent protein structure.

pronunciation IPA Transcription Poster
$14.99

A poster featuring the phonetic transcription of "pronunciation" in the International Phonetic Alphabet (IPA).

Pantothenic Acid Molecule Poster, Ball-and-Stick Model, English-Labeled
$19.99

A poster featuring the ball-and-stick model of the pantothenic acid molecule.

рукопись Morphemic Analysis Poster
$14.99

A poster featuring the morphemic analysis of the Russian word рукопись.

ABI to FASTA

Convert AB1 files (Applied Biosystems/ABI trace files) to FASTA format.

Sequence Alignment Viewer

Open FASTA files in the browser.

FASTA Editor

A simple online editor for biological sequences in the FASTA format.

FASTA Parse Tree Viewer

Visualize the parse tree of FASTA files.

GenBank to FASTA

Convert GenBank flat files to FASTA format.

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