Plasmid Map Generator
Create simple and clean plasmid maps from GenBank files.
1
LOCUS Exported 7700 bp ds-DNA circular SYN 23-MAR-20182
DEFINITION synthetic circular DNA3
ACCESSION .4
VERSION .5
KEYWORDS .6
SOURCE synthetic DNA construct7
ORGANISM synthetic DNA construct8
REFERENCE 1 (bases 1 to 7700)9
AUTHORS brady10
TITLE Direct Submission11
JOURNAL Exported Wednesday, Mar 10, 2021 from SnapGene 5.2.112
https://www.snapgene.com13
FEATURES Location/Qualifiers14
source 1..770015
/organism="synthetic DNA construct"16
/mol_type="other DNA"17
rep_origin 12..46718
/direction=RIGHT19
/label=f1 orim20
/note="f1 bacteriophage origin of replication; arrow 21
indicates direction of (+) strand synthesis"22
promoter 494..59823
/gene="bla"24
/label=AmpR promoter25
CDS 599..145926
/codon_start=127
/gene="bla"28
/product="beta-lactamase"29
/label=AmpR30
/note="confers resistance to ampicillin, carbenicillin, and31
related antibiotics"32
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI33
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS34
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW35
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA36
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS37
LIKHW"38
rep_origin 1630..221839
/direction=RIGHT40
/label=ori41
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 42
replication"43
misc_feature 2404..254644
/label=bom45
/note="basis of mobility region from pBR322"46
CDS complement(2648..2839)47
/codon_start=148
/gene="rop"49
/product="Rop protein, which maintains plasmids at low copy50
number"51
/label=rop52
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA53
DELYRSCLARFGDDGENL"54
CDS complement(3648..4730)55
/direction=RIGHT56
/codon_start=157
/gene="lacI"58
/product="lac repressor"59
/label=lacI60
/note="The lac repressor binds to the lac operator to 61
inhibit transcription in E. coli. This inhibition can be 62
relieved by adding lactose or 63
isopropyl-beta-D-thiogalactopyranoside (IPTG)."64
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL65
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV66
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH67
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA68
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC69
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR70
ALADSLMQLARQVSRLESGQ"71
promoter complement(4731..4808)72
/direction=RIGHT73
/gene="lacI"74
/label=lacI promoter75
promoter 5121..513976
/label=T7 promoter77
/note="promoter for bacteriophage T7 RNA polymerase"78
protein_bind 5140..516479
/label=lac operator80
/bound_moiety="lac repressor encoded by lacI"81
/note="The lac repressor binds to the lac operator to 82
inhibit transcription in E. coli. This inhibition can be 83
relieved by adding lactose or 84
isopropyl-beta-D-thiogalactopyranoside (IPTG)."85
CDS 5209..553586
/codon_start=187
/gene="trxA"88
/product="E. coli thioredoxin"89
/label=TrxA90
/translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE91
IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL92
DANLA"93
CDS 5557..557494
/codon_start=195
/product="6xHis affinity tag"96
/label=6xHis97
/translation="HHHHHH"98
primer_bind complement(5578..5628)99
/label=AviTag Insert R100
CDS 5584..5604101
/codon_start=1102
/product="tobacco etch virus (TEV) protease recognition and103
cleavage site"104
/label=TEV Site105
/translation="ENLYFQG"106
CDS 5611..5655107
/codon_start=1108
/product="peptide tag that allows for enzymatic 109
biotinylation"110
/label=AviTag(TM)111
/translation="GLNDIFEAQKIEWHE"112
primer_bind 5629..5673113
/label=AviTag Insert F114
CDS 5659..7503115
/codon_start=1116
/label=dsnPPR10-C2117
/translation="MGNDVVTYTTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNT118
LIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYTTLIDGLAKAGRLEEALCLFQEMKEK119
GVKPNVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLAKAGRLEE120
ALQLFQEMKEKGVKPSVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPSVVTYNTLI121
DGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGV122
KPDVVTYTTLIDGLAKAGRLEEALQLFQEMKEKGVKPNVVTYTTLIDGLAKAGRLEEAL123
QLFQEMKEKGVKPNVVTYNTLIDGLAKAGRLEEALCLFQEMKEKGVKPSVVTYNTLIDG124
LAKAGRLEEALQLFQEMKEKGVKPSVVTYTTLIDGLAKAGRLEEALQLFQEMKEKGVKP125
NVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLAKAGRLEEALQL126
FQEMKEKGVKPDVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLA127
KAGRLEEALQLFQEMKEKGVKPSVVTNNTLKDGASKAG"128
CDS 7544..7561129
/codon_start=1130
/product="6xHis affinity tag"131
/label=6xHis132
/translation="HHHHHH"133
terminator 7628..7675134
/label=T7 terminator135
/note="transcription terminator for bacteriophage T7 RNA 136
polymerase"137
ORIGIN138
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg139
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc140
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg141
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc142
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt143
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc144
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta145
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt146
481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta147
541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat148
601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt149
661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg150
721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga151
781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg152
841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt153
901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg154
961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg155
1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga156
1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc157
1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc158
1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc159
1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg160
1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac161
1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc162
1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt163
1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac164
1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa165
1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc166
1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt167
1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg168
1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc169
1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt170
1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga171
1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct172
2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg173
2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca174
2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa175
2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt176
2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga177
2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga178
2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg179
2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat180
2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct181
2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct182
2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct183
2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt184
2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg185
2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa186
2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc187
2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa188
3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta189
3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg190
3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag191
3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac192
3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca193
3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg194
3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc195
3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg196
3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca197
3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag198
3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt199
3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag200
3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc201
3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc202
3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct203
3901 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta204
3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg205
4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct206
4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga207
4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc208
4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg209
4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct210
4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt211
4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc212
4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc213
4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc214
4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact215
4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga216
4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc217
4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg218
4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag219
4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc220
4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat221
4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc222
5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat223
5101 cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa224
5161 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gagcgataaa225
5221 attattcacc tgactgacga cagttttgac acggatgtac tcaaagcgga cggggcgatc226
5281 ctcgtcgatt tctgggcaga gtggtgcggt ccgtgcaaaa tgatcgcccc gattctggat227
5341 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcaaaac228
5401 cctggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac229
5461 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc230
5521 gacgctaacc tggccggttc tggttctggc catatgcacc atcatcatca tcattcttct231
5581 ggtgagaatc tttattttca gggcgggtcc gggttgaatg atattttcga agcacagaaa232
5641 attgaatggc atgaggccat gggcaacgat gtggttacct acaccaccct gatcgatggt233
5701 ctggcgaaag cgggccgtct ggaggaagcg ctgcagctgt tccaagagat gaaggaaaaa234
5761 ggtgtgaagc cggacgtggt tacctataac accctgattg acggtctggc gaaggctggc235
5821 cgtctggagg aagcgctgca actgtttcag gaaatgaagg aaaaaggcgt taaaccggat236
5881 gttgtgacct ataccaccct gattgatggt ctggcgaagg ccggccgtct ggaggaagcg237
5941 ctgtgcctgt tccaggagat gaaagagaaa ggtgtgaagc cgaatgttgt gacctacaat238
6001 accctgatcg acggtctggc gaaggcaggc cgtctggagg aagcgctgca gttatttcag239
6061 gagatgaaag aaaaaggcgt taagccggat gttgttacct acaacacttt aattgacggt240
6121 ctggcgaaag ctggccgtct ggaggaagcg ctgcagctat ttcaggagat gaaggagaaa241
6181 ggtgtgaaac cgagcgtggt gacctacaac actttaatcg atggtctggc gaaggcgggc242
6241 cgtctggagg aagcgctgca gctttttcag gagatgaagg aaaaaggcgt taagccgagc243
6301 gtggttacct acaacactct tattgatggt ctggcgaaag ctggccgtct ggaggaagcg244
6361 ctgcagctct ttcaggagat gaaggaaaaa ggtgtgaaac cggatgttgt tacctataat245
6421 actttgatag atggtctggc gaaggcgggc cgtctggagg aagcgctgca gctattccag246
6481 gaaatgaaag agaaaggcgt taaaccggac gttgtgacct acaccacttt aattgatggt247
6541 ctggcgaaag ccggccgtct ggaggaagcg ctgcagctat tccaagaaat gaaagaaaaa248
6601 ggtgtgaagc ctaacgtggt tacctatacc actttaatcg acggtctggc gaaagccggc249
6661 cgtctggagg aagcgctgca gctattccaa gagatgaaag agaaaggcgt taagccgaat250
6721 gtggtgacct acaacacatt aattgatggt ctggcgaaag ccggccgtct ggaggaagcg251
6781 ctgtgtctgt tccaggagat gaaggaaaaa ggtgtgaagc cgagcgtggt gacctataat252
6841 actttgatcg atggtctggc gaaagcaggc cgtctggagg aagcgctgca gctatttcaa253
6901 gaaatgaaag aaaaaggcgt taagccgagc gtggtaacct ataccactct tatcgacggt254
6961 ctggcgaaag caggccgtct ggaggaagcg ctgcagctat ttcaagagat gaaagaaaaa255
7021 ggtgtgaaac ctaatgttgt gacctacaac actcttatcg atggtctggc gaaagcaggc256
7081 cgtctggagg aagcgctgca gctatttcaa gagatgaagg agaaaggcgt taagcctgat257
7141 gtggttacct ataatactct catcgatggt ctggcgaaag caggccgtct ggaggaagcg258
7201 ctgcagctat ttcaagagat gaaggaaaaa ggtgtgaagc cagatgttgt gacctataat259
7261 actctaatag atggtctggc gaaggcgggc cgtctggagg aagcgctgca gctatttcaa260
7321 gagatgaagg aaaaaggtgt taaaccggat gtggttacct acaacacact tattgatggt261
7381 ctggcgaaag caggccgtct ggaggaagcg ctgcagctat ttcaagagat gaaggaaaag262
7441 ggtgtgaaac cgagcgttgt gaccaataac accctgaagg acggcgcgag caaagcgggc263
7501 taagaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca264
7561 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga265
7621 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa266
7681 aggaggaact atatccggat267
//The GenBank file of the plasmid to be rendered.
The label of the plasmid to be rendered.
The rendered plasmid map.
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